Advanced Biotech Miscellaneous

Advanced Biotech Miscellaneous

Easy Questions

Advanced Biotech

Advanced Biotech Miscellaneous
  1. Match the following plant sources with their secondary metabolites and medical uses.
    ​​








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    Belladona plant produces a type of secondary metabolite called atropine which helps in the ocular examination procedure. Foxglove produces a secondary metabolite called digitalin that helps in the treatment of cardiac diseases. Pacific yew is another plant with medicinal use which produces taxol that is extensively used in cancer research and treatment purposes. Eucalyptus is used in the production of menthol that helps in the curing of cough and cold. ​

    Correct Option: A

    Belladona plant produces a type of secondary metabolite called atropine which helps in the ocular examination procedure. Foxglove produces a secondary metabolite called digitalin that helps in the treatment of cardiac diseases. Pacific yew is another plant with medicinal use which produces taxol that is extensively used in cancer research and treatment purposes. Eucalyptus is used in the production of menthol that helps in the curing of cough and cold. ​

  1. The plasmid DNA was subjected to restriction digestion using the enzyme EcoRI and analysed on an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the DNA bands shown in the image in Group I with their identity in Group II.

    ​
    Group–I​  ​​ Group–II
    P.​ Bands labeled as A​ 1.​ Nicked
    Q.​ Band labeled as B​ 2.​ Supercoiled
    R.​ Band labeled as C​ 3.​ Concatemers
    S.​ Band labeled as D​ 4.​ Linear
    ​​








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    The bands which are labeled as A were uncut and separated at three different regions on the agarose gel after digestion with EcoRI. It shows that the plasmid A separates is having concatemers. Plasmid B labeled as B is nicked while Plasmid C which is labeled as C has a very thick band which suggests that Plasmid C is supercoiled. Band D was the only plasmid which was the digested product of Eco RI are linear because EcoRI has already digested the plasmid.

    Correct Option: A

    The bands which are labeled as A were uncut and separated at three different regions on the agarose gel after digestion with EcoRI. It shows that the plasmid A separates is having concatemers. Plasmid B labeled as B is nicked while Plasmid C which is labeled as C has a very thick band which suggests that Plasmid C is supercoiled. Band D was the only plasmid which was the digested product of Eco RI are linear because EcoRI has already digested the plasmid.

  1. The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to ​​








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    Serum is commonly used as a supplement to cell culture media. It supports cell growth and product formation by providing a broad spectrum of macromolecules, nutrients and vitamins as well as certain growth factors and hormones. However, serum must be filter sterilized with 0.45? filter paper before using in the media to grow mammalian cells because it contains Mycoplasma and microorganisms as contaminants. ​

    Correct Option: C

    Serum is commonly used as a supplement to cell culture media. It supports cell growth and product formation by providing a broad spectrum of macromolecules, nutrients and vitamins as well as certain growth factors and hormones. However, serum must be filter sterilized with 0.45? filter paper before using in the media to grow mammalian cells because it contains Mycoplasma and microorganisms as contaminants. ​

  1. The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be ​​








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    The number of bases present in the restriction site of the restriction enzyme = 6
    ​​We know, there are four different types of nitrogenous bases in a DNA molecule. ​​
    So, the statistical frequency of occurrence of the restriction site would be = 46 = 4096

    Correct Option: D

    The number of bases present in the restriction site of the restriction enzyme = 6
    ​​We know, there are four different types of nitrogenous bases in a DNA molecule. ​​
    So, the statistical frequency of occurrence of the restriction site would be = 46 = 4096

  1. Plasmid DNA (0.5 µg) containing an ampicillin resistance marker was added to 200 µl of competent cells. The transformed competent cells were diluted 10,000 times, out of which 50 µl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is _____ ×  106 cfu. µg​– 1.








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    DNA​= 0.5 µg

    Dillution =
    50
    		 = 2.5 × 10-5
    200 × 10000

    TE =
    Number of colonies
    Dilution × Amount of DNA

    =
    35
    		 = 2.8 × 106
    2.5 × 10-5 × 0.5

    Correct Option: C

    DNA​= 0.5 µg

    Dillution =
    50
    		 = 2.5 × 10-5
    200 × 10000

    TE =
    Number of colonies
    Dilution × Amount of DNA

    =
    35
    		 = 2.8 × 106
    2.5 × 10-5 × 0.5