Gene therapy is the therapeutic delivery of nucleic acid polymers into a patient’s cells as a drug to treat disease. Avidin is a protein derived from both avians and amphibians that shows considerable affinity for biotin, a co-factor that plays a role in multiple eukaryotic biological processes.

Correct Option: D

Gene therapy is the therapeutic delivery of nucleic acid polymers into a patient’s cells as a drug to treat disease. Avidin is a protein derived from both avians and amphibians that shows considerable affinity for biotin, a co-factor that plays a role in multiple eukaryotic biological processes.

Gene therapy is the therapeutic delivery of nucleic acid polymers into a patient’s cells as a drug to treat disease. Avidin is a protein derived from both avians and amphibians that shows considerable affinity for biotin, a co-factor that plays a role in multiple eukaryotic biological processes. only be used to obtain a viability count, not a total cell count (cells/mL).  The viability will be determined by the trypan blue assay , and it can be used with the total cell count (obtained from a Coulter counter) to determine a total count of viable cells, as : Number of viable cells = (% viability)*(Total number of cells) ​​

Correct Option: D

Gene therapy is the therapeutic delivery of nucleic acid polymers into a patient’s cells as a drug to treat disease. Avidin is a protein derived from both avians and amphibians that shows considerable affinity for biotin, a co-factor that plays a role in multiple eukaryotic biological processes. only be used to obtain a viability count, not a total cell count (cells/mL).  The viability will be determined by the trypan blue assay , and it can be used with the total cell count (obtained from a Coulter counter) to determine a total count of viable cells, as : Number of viable cells = (% viability)*(Total number of cells) ​​

Secondary treatment is the portion of a sewage treatment sequence removing dissolved and colloidal compounds measured as BOD. Secondary treatment is traditionally applied to the liquid portion of sewage after primary treatment has removed settleable solids and floating material. Secondary treatment is typically performed by indigenous, aquatic microorganisms in a managed aerobic habitat. Bacteria and protozoa consume biodegradable soluble organic contaminants (e.g. sugars, fats, and organic short-chain carbon molecules from human waste, food waste, soaps and detergent) while reproducing to form cells of biological solids.

Correct Option: B

Secondary treatment is the portion of a sewage treatment sequence removing dissolved and colloidal compounds measured as BOD. Secondary treatment is traditionally applied to the liquid portion of sewage after primary treatment has removed settleable solids and floating material. Secondary treatment is typically performed by indigenous, aquatic microorganisms in a managed aerobic habitat. Bacteria and protozoa consume biodegradable soluble organic contaminants (e.g. sugars, fats, and organic short-chain carbon molecules from human waste, food waste, soaps and detergent) while reproducing to form cells of biological solids.

The T-DNA transfer process of Agrobacterium tumefaciens is activated by the induction of the expression of the Ti plasmid virulence loci by plant signal molecules such as acetosyringone. The overdrive  proteins from acetosyringone-induced Agrobacterium cells interact with T-DNA border and overdrive sequences. The vir gene products act in trans to mobilize the T-DNA element from the bacterial Ti plasmid. The T-DNA is bounded by 25-base pair direct repeat sequences, which are the only sequences on the element essential for transfer. Thus, specific reactions must occur at the border sites to generate a transferable T-DNA copy.

Correct Option: B

The T-DNA transfer process of Agrobacterium tumefaciens is activated by the induction of the expression of the Ti plasmid virulence loci by plant signal molecules such as acetosyringone. The overdrive  proteins from acetosyringone-induced Agrobacterium cells interact with T-DNA border and overdrive sequences. The vir gene products act in trans to mobilize the T-DNA element from the bacterial Ti plasmid. The T-DNA is bounded by 25-base pair direct repeat sequences, which are the only sequences on the element essential for transfer. Thus, specific reactions must occur at the border sites to generate a transferable T-DNA copy.

Bicarbonate is often included to act as a buffer system in a conduction with the CO₂ environment (5-10%) in which the cell should be cultured. This allows the cultures to be maintained at the normally optimum pH range of 6.9 - 7.4. The CO₂ is provided by a controlled atmosphere inside an incubator or alternatively in a sealed flask. ​

Correct Option: A

Bicarbonate is often included to act as a buffer system in a conduction with the CO₂ environment (5-10%) in which the cell should be cultured. This allows the cultures to be maintained at the normally optimum pH range of 6.9 - 7.4. The CO₂ is provided by a controlled atmosphere inside an incubator or alternatively in a sealed flask. ​