Advanced Biotech Miscellaneous
- Match the items in groupl with the terms given in group 2
Group 1 Group 2 P. Lactobacillus and Bifidobacteria 1. Prebiotics Q. Polychlorobenzenes 2. Probiotics (PCBs) R. Fructo-oligosaccharides 3. Antibiotics S. β-Lactams 4. Xenobiotics
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⚈ Probiotics are live bacteria that may confer a health benefit on the host. Lactic acid bacteria (LAB) and bifidobacteria are the most common types of microbes used as probiotics.
⚈ A xenobiotic is a chemical which is found in an organism but which is not normally produced or expected to be present in it. The term xenobiotics is very often used in the context of pollutants such as dioxins and polychlorinated biphenyls.
⚈ Fructo-oligosaccharide (FOS) and lactulose have been classified as prebiotics. Prebiotics are non-digestible food ingredients that stimulate the growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial to health.
⚈ β-Lactam antibiotics (beta-lactam antibiotics) are a broad class of antibiotics, consisting of all antibiotic agents that contains a β-lactam ring in their molecular structures.
Correct Option: A
⚈ Probiotics are live bacteria that may confer a health benefit on the host. Lactic acid bacteria (LAB) and bifidobacteria are the most common types of microbes used as probiotics.
⚈ A xenobiotic is a chemical which is found in an organism but which is not normally produced or expected to be present in it. The term xenobiotics is very often used in the context of pollutants such as dioxins and polychlorinated biphenyls.
⚈ Fructo-oligosaccharide (FOS) and lactulose have been classified as prebiotics. Prebiotics are non-digestible food ingredients that stimulate the growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial to health.
⚈ β-Lactam antibiotics (beta-lactam antibiotics) are a broad class of antibiotics, consisting of all antibiotic agents that contains a β-lactam ring in their molecular structures.
- A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 µl reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in O.1µl of reaction ?
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In PCR, the template DNA after ‘n’ cycle = (original DNA) × 2n
= 400 × 220
But, this is amount in 100µl. In 0.1 µl we have,= 400 × 220 × 0.1 = 4.194304 × 105 100 Correct Option: D
In PCR, the template DNA after ‘n’ cycle = (original DNA) × 2n
= 400 × 220
But, this is amount in 100µl. In 0.1 µl we have,= 400 × 220 × 0.1 = 4.194304 × 105 100
- Which of the following reagents is used for harvesting anchorage-dependent animal cells from culture vessels ?
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Cells are harvested when the cells have reached a population density which suppresses growth. For adherent cultures, we use trypsin or collagenase. Trypsin, collagenase, or pronase, usually in combination with EDTA, causes cells to detach from the growth surface, thereby aiding in harvesting of anchorage dependent animal cells from culture vessels.
Correct Option: A
Cells are harvested when the cells have reached a population density which suppresses growth. For adherent cultures, we use trypsin or collagenase. Trypsin, collagenase, or pronase, usually in combination with EDTA, causes cells to detach from the growth surface, thereby aiding in harvesting of anchorage dependent animal cells from culture vessels.
- Oils rich in PUFA are NOT desirable for bio-diesel production because
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PUFAs are long-chain fatty acids containing two or more double bonds.
They provide structural & functional characteristics, and are involved in a wide range of biological components including membranes (in phospholipids). Equally important PUFAs serve as precursors for conversion into metabolites that regulate critical biological functions.Biodiesel is a non-petroleum and environmentally-friendly alternative to petroleum-based diesel fuel and not fatty acid.PUFA is fatty acid it is not used as it forms epoxide in presence of oxygen.Correct Option: A
PUFAs are long-chain fatty acids containing two or more double bonds.
They provide structural & functional characteristics, and are involved in a wide range of biological components including membranes (in phospholipids). Equally important PUFAs serve as precursors for conversion into metabolites that regulate critical biological functions.Biodiesel is a non-petroleum and environmentally-friendly alternative to petroleum-based diesel fuel and not fatty acid.PUFA is fatty acid it is not used as it forms epoxide in presence of oxygen.
- Match the following marker genes in group 1 with suitable selecting agent in group 2
Group 1 Group 2 P. npt II 1. Glyphosate Q. aroA 2. Phosphinothricin R. hpt 3. Kanamycin S. bar 4. Hygromycin B
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Correct Option: D