Advanced Biotech Miscellaneous Easy Questions and Answers | Page - 33

Advanced Biotech Miscellaneous

In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be

1. 0
1. < 1
1. 1
1. > 1

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About ddNTP_s = 1
dNTP_s 300

Correct Option: B

About ddNTP_s = 1
dNTP_s 300

Which one of the following features is NOT required in a prokaryotic expression vector ?

1. oriC
1. Selection marker
1. CMV promoter
1. Ribosome binding site

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CMV promoter is plant expression vector.

Correct Option: C

CMV promoter is plant expression vector.

In animal cell culture, a CO₂ enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?

1. Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO₂
1. Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO₂
1. Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO₂
1. CO₂ requirement is independent of bicarbonate concentration in the medium

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Bicarbonate is often included to act as a buffer system in a conduction with the CO₂ environment (5-10%) in which the cell should be cultured. This allows the cultures to be maintained at the normally optimum pH range of 6.9 - 7.4. The CO₂ is provided by a controlled atmosphere inside an incubator or alternatively in a sealed flask.

Correct Option: A

Bicarbonate is often included to act as a buffer system in a conduction with the CO₂ environment (5-10%) in which the cell should be cultured. This allows the cultures to be maintained at the normally optimum pH range of 6.9 - 7.4. The CO₂ is provided by a controlled atmosphere inside an incubator or alternatively in a sealed flask.

A variety of genetic elements are used in the transgenic plant research. Match the genetic elements (Column-I) with their corresponding source (Column-II).
Column-I 
P. Ubiquitin1 promoter
Q. Nos transcriptional terminator
R. bar selection marker gene
S. gus reporter gene
Column-II 
1. Agrobacterium tumefaciens
2. Streptomyces hygroscopicus
3. Escherichia coli
4. Zea mays

1. P-2 , Q-1 , R-3 , S-4
1. P-2 , Q-3 , R-4 , S-1
1. P-3 , Q-4 , R-1 , S-2
1. P-4 , Q-1 , R-2 , S-3

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Certain genetic elements used in transgenic research includes Ubiquitin I promoter, Nos Transcriptional terminator, bar Selection Marker Gene, gus Reporter gene. They are obtained from Zea mays, Agrobacterium tumifaciens, Streptomyces hygroscopicus, Escherichia coli respectively.

Correct Option: D

Certain genetic elements used in transgenic research includes Ubiquitin I promoter, Nos Transcriptional terminator, bar Selection Marker Gene, gus Reporter gene. They are obtained from Zea mays, Agrobacterium tumifaciens, Streptomyces hygroscopicus, Escherichia coli respectively.

Match the secondary metabolites (Column-I) with the corresponding plant species (Column-II).
Column-I Column-II
P. Morphine 1. Datura stramonium
Q. Pyrethrins 2. Catharanthus roseus
R. Scopolamine 3. Papaver somniferum
S. Vincristine 4. Tagetes erecta

1. P-4 , Q-3 , R-1 , S-2
1. P-3 , Q-4 , R-1 , S-2
1. P-2 , Q-3 , R-4 , S-1
1. P-4 , Q-1 , R-2 , S-3

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Secondary Metabolites including Morphine, Pyrethrins, Scopolamine, Vincristine are extracted from Papaver somniferum, Tagetes erecta, Datura stramonium, Catharanthus roses respectively.

Correct Option: B

Secondary Metabolites including Morphine, Pyrethrins, Scopolamine, Vincristine are extracted from Papaver somniferum, Tagetes erecta, Datura stramonium, Catharanthus roses respectively.