Advanced Biotech Miscellaneous
- Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size 4.3 × 105 bp with AluI(5′-AG↓CT-3′) is ______ × 103.
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Average number of fragments generated = 
1 × 1 × 1 × 1 
× 4.3 × 105 4 4 4 4 = 4.3 × 105 = 1.68 × 103 256 Correct Option: B
Average number of fragments generated = 1 × 1 × 1 × 1 × 4.3 × 105 4 4 4 4 = 4.3 × 105 = 1.68 × 103 256
- A linear double stranded DNA of length 8 kbp has three restriction sites. Each of these can either be a BamHi or a HaeIII site. The DNA was digested completely with both enzymes. The products were purified and subjected to an end-filling reaction using the Klenow fragment and [α– 32P]-dCTP. The products of the end-filling reaction were purified, resolved by electrophoresis, stained with ethidium bromide (EtBr) and then subjected to autoradiography. The corresponding images are shown below.
The numbers below each band in the sample lane in the autoradiograph represent their mean signal intensity in arbitrary units. Which one of the following options is the correct restriction map of the DNA ?
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Hae III : GG|CC
CC | GG
BamHI : G | GATC
CCTAG | G Correct Option: A
Hae III : GG|CC
CC | GG
BamHI : G | GATC
CCTAG | G
- Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5′ –gacctgtgg------------------atacggGat –3′
3′ –ctggacacc-------------------tatgcccta –5′
Primers
P. 5′ –gacctgtgg–3′
Q. 5′ –ccacaggtc–3′
R. 5′ –tagggcata–3′
S. 5′ –atcccgtat–3′
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Respective primers will be anneal to 3′ ends of the two template strands; keeping antiparallel nature of DNA duplex and complimentarity in the mind.
Correct Option: B
Respective primers will be anneal to 3′ ends of the two template strands; keeping antiparallel nature of DNA duplex and complimentarity in the mind.
- Which one of the following is a second generation genetically engineered crop ?
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2nd generation crop are the value added crops i.e; increased nutritional value. Instead of insect resistance (Bt) and herbicide tolerance (HT).
Correct Option: C
2nd generation crop are the value added crops i.e; increased nutritional value. Instead of insect resistance (Bt) and herbicide tolerance (HT).
- Which one of the following is NOT used for the measurement of cell viability in animal cell culture ?
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Gene therapy is the therapeutic delivery of nucleic acid polymers into a patient’s cells as a drug to treat disease. Avidin is a protein derived from both avians and amphibians that shows considerable affinity for biotin, a co-factor that plays a role in multiple eukaryotic biological processes. only be used to obtain a viability count, not a total cell count (cells/mL). The viability will be determined by the trypan blue assay , and it can be used with the total cell count (obtained from a Coulter counter) to determine a total count of viable cells, as : Number of viable cells = (% viability)*(Total number of cells)
Correct Option: D
Gene therapy is the therapeutic delivery of nucleic acid polymers into a patient’s cells as a drug to treat disease. Avidin is a protein derived from both avians and amphibians that shows considerable affinity for biotin, a co-factor that plays a role in multiple eukaryotic biological processes. only be used to obtain a viability count, not a total cell count (cells/mL). The viability will be determined by the trypan blue assay , and it can be used with the total cell count (obtained from a Coulter counter) to determine a total count of viable cells, as : Number of viable cells = (% viability)*(Total number of cells)
