Advanced Biotech Miscellaneous

Advanced Biotech Miscellaneous

Easy Questions

Advanced Biotech

Advanced Biotech Miscellaneous
  1. Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size 4.3 × 10​5 bp with AluI(5′-AG↓CT-3′) is ______ × 10​3. ​








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    Average number of fragments generated =
    1
    		 ×
    1
    		 ×
    1
    		 ×
    1
    		 × 4.3 × 105
    4444

    =
    4.3 × 105
    		 = 1.68 × 103
    256

    Correct Option: B

    Average number of fragments generated =
    1
    		 ×
    1
    		 ×
    1
    		 ×
    1
    		 × 4.3 × 105
    4444

    =
    4.3 × 105
    		 = 1.68 × 103
    256

  1. A linear double stranded DNA of length 8 kbp has three restriction sites. Each of these can either be a BamHi or a HaeIII site. The DNA was digested completely with both enzymes. The products were purified and subjected to an end-filling reaction using the Klenow fragment and [α​– 32P]-dCTP. The products of the end-filling reaction were purified, resolved by electrophoresis, stained with ethidium bromide (EtBr) and then subjected to autoradiography. The corresponding images are shown below.

    The numbers below each band in the sample lane in the autoradiograph represent their mean signal intensity in arbitrary units. Which one of the following options is the correct restriction map of the DNA ?








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    Hae III : GG|CC ​​​ 
        CC | GG ​​
    BamHI :      G | GATC ​​​ 
          CCTAG | G ​

    Correct Option: A

    Hae III : GG|CC ​​​ 
        CC | GG ​​
    BamHI :      G | GATC ​​​ 
          CCTAG | G ​

  1. Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR). ​​
    5′ –gacctgtgg------------------atacggGat –3′ ​​
    3′ –ctggacacc-------------------tatgcccta –5′ ​​
    Primers
    ​​P.​ 5′ –gacctgtgg–3′ ​​
    Q. ​5′ –ccacaggtc–3′ ​​
    R.​ 5′ –tagggcata–3′
    S.​ 5′ –atcccgtat–3′ ​








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    Respective primers will be anneal to 3′ ends of the two template strands; keeping antiparallel nature of DNA duplex and complimentarity in the mind.

    Correct Option: B

    Respective primers will be anneal to 3′ ends of the two template strands; keeping antiparallel nature of DNA duplex and complimentarity in the mind.

  1. Which one of the following is a second generation genetically engineered crop ?








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    2nd generation crop are the value added crops i.e; increased nutritional value. Instead of insect resistance (Bt) and herbicide tolerance (HT). ​

    Correct Option: C

    2nd generation crop are the value added crops i.e; increased nutritional value. Instead of insect resistance (Bt) and herbicide tolerance (HT). ​

  1. ​Which one of the following is NOT a product of denitrification in Pseudomonas? ​








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    NH4+ product is only in anaerobic processes like waste water treatment.

    Correct Option: D

    NH4+ product is only in anaerobic processes like waste water treatment.