Advanced Biotech Miscellaneous

Advanced Biotech Miscellaneous

Easy Questions

Advanced Biotech

Advanced Biotech Miscellaneous
  1. Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
    ​​Assertion :​ The production of secondary metabolites in plant cell cultures is enhanced by the addition of elicitors. ​​
    Reason : Elicitors induce the expression of enzymes responsibie for the biosynthesis of secondary metabolites. ​​








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    Elicitors are compounds, which activate chemical defense in plants. Various biosynthetic biosynthetic pathways are activated in treated plants depending on the compound used. They are compounds that when introduced into a living organism signals the activation or synthesis of another compound. Thus (R) is true but (A) is false. ​

    Correct Option: D

    Elicitors are compounds, which activate chemical defense in plants. Various biosynthetic biosynthetic pathways are activated in treated plants depending on the compound used. They are compounds that when introduced into a living organism signals the activation or synthesis of another compound. Thus (R) is true but (A) is false. ​

  1. A single base pair of DNA weighs 1.1 × 10–21 grains. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 ug of purified DNA?








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    Given, weight of single base pair of DNA​​​= 1.1 * 10–21 grains ​​
    Length of plasmid vector= 2750bp ​​
    Purified DNA= 1ug ​​
    Thus, 0.55 picomoles of a plasmid vector of length 2750bp are contained in 1ug of purified DNA.

    Correct Option: B

    Given, weight of single base pair of DNA​​​= 1.1 * 10–21 grains ​​
    Length of plasmid vector= 2750bp ​​
    Purified DNA= 1ug ​​
    Thus, 0.55 picomoles of a plasmid vector of length 2750bp are contained in 1ug of purified DNA.

  1. Which one of the following is NOT a protoplast fusion inducing agent? ​​








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    Fusion of freely isolated protoplasts from different sources with the help of fusion inducing chemical agents is known as induced fusion. It includes use of high pH or Ca++ treatment, Use of PEG (Polyethylene glycol) which induces protoplast aggregation and subsequent fusion and the use of inactivated Sendai virus for fusion which occur in four temperature-dependent stages. ​​
    Hence Colchicine is not a protoplast fusion inducing agent. Colchicine is used to prevent gout attacks in adults, and to relieve the pain of gout attacks when they occur. Colchicine is also used to treat familial Mediterranean fever (FMF; an inborn condition that causes episodes of fever, pain, and swelling of the stomach area, lungs, and joints) in adults and children 4 years of age and older. ​Fusion of freely isolated protoplasts from different sources with the help of fusion inducing chemical agents is known as induced fusion. It includes use of high pH or Ca++ treatment, Use of PEG (Polyethylene glycol) which induces protoplast aggregation and subsequent fusion and the use of inactivated Sendai virus for fusion which occur in four temperature-dependent stages. ​​
    Hence Colchicine is not a protoplast fusion inducing agent. Colchicine is used to prevent gout attacks in adults, and to relieve the pain of gout attacks when they occur. Colchicine is also used to treat familial Mediterranean fever (FMF; an inborn condition that causes episodes of fever, pain, and swelling of the stomach area, lungs, and joints) in adults and children 4 years of age and older. ​

    Correct Option: D

    Fusion of freely isolated protoplasts from different sources with the help of fusion inducing chemical agents is known as induced fusion. It includes use of high pH or Ca++ treatment, Use of PEG (Polyethylene glycol) which induces protoplast aggregation and subsequent fusion and the use of inactivated Sendai virus for fusion which occur in four temperature-dependent stages. ​​
    Hence Colchicine is not a protoplast fusion inducing agent. Colchicine is used to prevent gout attacks in adults, and to relieve the pain of gout attacks when they occur. Colchicine is also used to treat familial Mediterranean fever (FMF; an inborn condition that causes episodes of fever, pain, and swelling of the stomach area, lungs, and joints) in adults and children 4 years of age and older. ​

  1. ​Protein-DNA interactions in vivo can be studied by








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    The strength of ChIP assays is their ability to capture a snapshot of specific protein: DNA interactions occurring in a system and to quantitate the interactions using quantitative polymerase chain reaction (qPCR).

    Correct Option: C

    The strength of ChIP assays is their ability to capture a snapshot of specific protein: DNA interactions occurring in a system and to quantitate the interactions using quantitative polymerase chain reaction (qPCR).

  1. Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because ​​








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    DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury.

    Correct Option: C

    DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury.