Advanced Biotech Miscellaneous


Advanced Biotech Miscellaneous

  1. Protein-protein interactions are studied by ​​
    P.​ DNA foot printing ​​
    Q. ​Yeast two hybrid system ​​
    R. ​Ligase chain reaction
    ​​S.​ Mass spectrometry ​









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    ​Yeast two hybrid screening is a molecular biology technique used to discover protein-protein interaction and protein-DNA interaction by testing for physical interactions. Mass spectrometry (MS) is an analytical technique that measures the mass-to-charge ratio of charged particles.

    Correct Option: B

    ​Yeast two hybrid screening is a molecular biology technique used to discover protein-protein interaction and protein-DNA interaction by testing for physical interactions. Mass spectrometry (MS) is an analytical technique that measures the mass-to-charge ratio of charged particles.


  1. ​The study of evolutionary relationships is known as ​​









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    Phylogenetics deals with evolutionary relatedness of the species. It calculates the distances amongst the species from its origin and can track down any ancestral relationship.

    Correct Option: C

    Phylogenetics deals with evolutionary relatedness of the species. It calculates the distances amongst the species from its origin and can track down any ancestral relationship.



  1. Restriction endonucleases which recognize and cut same recognition sequences are known as









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    Restriction endonucleases that recognize the same sequence are isoschizomers. The first example discovered is called a prototype and all subsequent enzymes that recognize the same sequence are isoschizomers of the prototype. For example, Sph I (CGTAC/G) and Bbu I (CGTAC/G) are isoschizomers of each other. Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. ​

    Correct Option: A

    Restriction endonucleases that recognize the same sequence are isoschizomers. The first example discovered is called a prototype and all subsequent enzymes that recognize the same sequence are isoschizomers of the prototype. For example, Sph I (CGTAC/G) and Bbu I (CGTAC/G) are isoschizomers of each other. Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. ​


  1. ​‘Hairy roots’ induced in vitro by the infection of Agrobacterium rhizogenes, are characterized by ​​
    P.​ a high degree of lateral branching ​​
    Q.​ genetic instability of culture ​​
    R.​ an absence of geotropism ​​
    S. ​poor biomass production









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    Hairy roots are produced by infecting sterile plant with a natural genetic engineer, Agrobacterim rhizogenes. Genes for auxin synthesis and sensitivity are engineered into plant cells leading to gravity- insensitive mass root production. It is very useful for products produced in roots. Aggregation and shear sensitivity are a major problem for scale up. ​

    Correct Option: A

    Hairy roots are produced by infecting sterile plant with a natural genetic engineer, Agrobacterim rhizogenes. Genes for auxin synthesis and sensitivity are engineered into plant cells leading to gravity- insensitive mass root production. It is very useful for products produced in roots. Aggregation and shear sensitivity are a major problem for scale up. ​



  1. Shoot organogenesis by tissue culture results into









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    Shoot organogenesis is one of the in vitro plant regeneration pathway. It has been widely employed in plant biotechnology for invitro micropropagation and genetic transformation, as well as in study of plant development. Organogenesis continues until the definitive characteristics of the organs or plant parts are achieved.

    Correct Option: B

    Shoot organogenesis is one of the in vitro plant regeneration pathway. It has been widely employed in plant biotechnology for invitro micropropagation and genetic transformation, as well as in study of plant development. Organogenesis continues until the definitive characteristics of the organs or plant parts are achieved.