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Advanced Biotech Miscellaneous

  1. A linear double stranded DNA of length 8 kbp has three restriction sites. Each of these can either be a BamHi or a HaeIII site. The DNA was digested completely with both enzymes. The products were purified and subjected to an end-filling reaction using the Klenow fragment and [α​– 32P]-dCTP. The products of the end-filling reaction were purified, resolved by electrophoresis, stained with ethidium bromide (EtBr) and then subjected to autoradiography. The corresponding images are shown below.

    The numbers below each band in the sample lane in the autoradiograph represent their mean signal intensity in arbitrary units. Which one of the following options is the correct restriction map of the DNA ?



Correct Option: A

Hae III : GG|CC ​​​ 
    CC | GG ​​
BamHI :      G | GATC ​​​ 
      CCTAG | G ​



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