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Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5′ –gacctgtgg------------------atacggGat –3′
3′ –ctggacacc-------------------tatgcccta –5′
Primers
P. 5′ –gacctgtgg–3′
Q. 5′ –ccacaggtc–3′
R. 5′ –tagggcata–3′
S. 5′ –atcccgtat–3′
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- P and R
- P and S
- Q and R
- Q and S
Correct Option: B
Respective primers will be anneal to 3′ ends of the two template strands; keeping antiparallel nature of DNA duplex and complimentarity in the mind.
