In animal cell culture, a CO2 enriched atmosphere in the

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In animal cell culture, a CO₂ enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?

1. Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO₂
2. Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO₂
3. Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO₂
4. CO₂ requirement is independent of bicarbonate concentration in the medium

Correct Option: A

Bicarbonate is often included to act as a buffer system in a conduction with the CO₂ environment (5-10%) in which the cell should be cultured. This allows the cultures to be maintained at the normally optimum pH range of 6.9 - 7.4. The CO₂ is provided by a controlled atmosphere inside an incubator or alternatively in a sealed flask.