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Which of the following reagents is used for harvesting anchorage-dependent animal cells from culture vessels ?
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- Trypsin/Collagensase
- Trypsin/Collagen
- Collagen/Fibronectin
- DMSO
Correct Option: A
Cells are harvested when the cells have reached a population density which suppresses growth. For adherent cultures, we use trypsin or collagenase. Trypsin, collagenase, or pronase, usually in combination with EDTA, causes cells to detach from the growth surface, thereby aiding in harvesting of anchorage dependent animal cells from culture vessels.