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Plasmid DNA (0.5 µg) containing an ampicillin resistance marker was added to 200 µl of competent cells. The transformed competent cells were diluted 10,000 times, out of which 50 µl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is _____ × 106 cfu. µg– 1.
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- 0.6
- 1.4
- 2.8
- 4.8
Correct Option: C
DNA= 0.5 µg
| Dillution = | = 2.5 × 10-5 | |
| 200 × 10000 |
| TE = | ||
| Dilution × Amount of DNA |
| = | = 2.8 × 106 | |
| 2.5 × 10-5 × 0.5 |
