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The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to
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- remove large proteins
- remove collagen only
- remove mycoplasma and microorganisms
- remove foaming agents
Correct Option: C
Serum is commonly used as a supplement to cell culture media. It supports cell growth and product formation by providing a broad spectrum of macromolecules, nutrients and vitamins as well as certain growth factors and hormones. However, serum must be filter sterilized with 0.45? filter paper before using in the media to grow mammalian cells because it contains Mycoplasma and microorganisms as contaminants.
