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The plasmid DNA was subjected to restriction digestion using the enzyme EcoRI and analysed on an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the DNA bands shown in the image in Group I with their identity in Group II.
Group–I Group–II P. Bands labeled as A 1. Nicked Q. Band labeled as B 2. Supercoiled R. Band labeled as C 3. Concatemers S. Band labeled as D 4. Linear
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- P-3 , Q-1 , R-2 , S-4
- P-1 , Q-4 , R-3 , S-2
- P-4 , Q-3 , R-1 , S-2
- P-4 , Q-1 , R-2 , S-3
Correct Option: A
The bands which are labeled as A were uncut and separated at three different regions on the agarose gel after digestion with EcoRI. It shows that the plasmid A separates is having concatemers. Plasmid B labeled as B is nicked while Plasmid C which is labeled as C has a very thick band which suggests that Plasmid C is supercoiled. Band D was the only plasmid which was the digested product of Eco RI are linear because EcoRI has already digested the plasmid.
