Biochemical Miscellaneous


Biochemical Miscellaneous

  1. Which one of the following monolayer culture systems have the highest surface area: medium ratio? ​









  1. View Hint View Answer Discuss in Forum

    ​Hollow fibers also provide a tremendous amount of surface area in a small volume. Cells grow on and around the fibers at densities of greater than 1 x 108 per mL.
    Hollow fiber cell culture is the only means to culture cells at in vivo like cell densities.

    Correct Option: C

    ​Hollow fibers also provide a tremendous amount of surface area in a small volume. Cells grow on and around the fibers at densities of greater than 1 x 108 per mL.
    Hollow fiber cell culture is the only means to culture cells at in vivo like cell densities.


  1. Power number, also called Newton's number, is defined as a dimensionless parameter relating to









  1. View Hint View Answer Discuss in Forum

    The power number, NP is a commonly-used dimensionless number relating the resistance force to the intertia force. ​​The power number has different specifications according to the field of application e.g., for stirrers, the power number is defined as :

    NP =
    P
    with
    ρn3d5

    P = power ​​
    ρ = fluid density
    ​​n = rotational speed ​​
    d = diameter of stirrer ​

    Correct Option: C

    The power number, NP is a commonly-used dimensionless number relating the resistance force to the intertia force. ​​The power number has different specifications according to the field of application e.g., for stirrers, the power number is defined as :

    NP =
    P
    with
    ρn3d5

    P = power ​​
    ρ = fluid density
    ​​n = rotational speed ​​
    d = diameter of stirrer ​



  1. The selection of the appropriate purification method in the product recovery after microbial fermentation depends on the ​​









  1. View Hint View Answer Discuss in Forum

    The degree of protein purity required depends on the intended end use of the protein. For some applications, a crude extract is sufficient. However, for other uses, such as in foods and pharmaceuticals, a high level of purity is required. In order to achieve this, several protein purification methods are typically used, in a series of purification steps. Protein purification step also differs for intra-cellular and extra-cellular products.

    Correct Option: D

    The degree of protein purity required depends on the intended end use of the protein. For some applications, a crude extract is sufficient. However, for other uses, such as in foods and pharmaceuticals, a high level of purity is required. In order to achieve this, several protein purification methods are typically used, in a series of purification steps. Protein purification step also differs for intra-cellular and extra-cellular products.


  1. The precursor for penicillin-G biosynthesis during fermentation process is









  1. View Hint View Answer Discuss in Forum

    Penicillium chrysogenum utilizes phenylacetic acid as a side chain precursor in penicillin G biosynthesis. During industrial production of penicillin G, phenylacetic acid is fed in small amounts to the medium to avoid toxic side effects. Phenylacetic acid is taken up from the medium and intracellularly coupled to 6-aminopenicillanic acid.

    Correct Option: A

    Penicillium chrysogenum utilizes phenylacetic acid as a side chain precursor in penicillin G biosynthesis. During industrial production of penicillin G, phenylacetic acid is fed in small amounts to the medium to avoid toxic side effects. Phenylacetic acid is taken up from the medium and intracellularly coupled to 6-aminopenicillanic acid.



  1. ​In large scale fermentation process, air is sterilized by









  1. View Hint View Answer Discuss in Forum

    To prevent contamination of either the fermentation by air-borne microorganisms or the environment by aerosols generated within the fermenter, both air input and air exhaust ports have air filters such as glass fibre filters, mineral fibers filters.

    Correct Option: D

    To prevent contamination of either the fermentation by air-borne microorganisms or the environment by aerosols generated within the fermenter, both air input and air exhaust ports have air filters such as glass fibre filters, mineral fibers filters.