Biochemical Miscellaneous Easy Questions and Answers

Biochemical Miscellaneous

The kinetics of the disintegration of baker’s yeast cells in a bead mill is described as dp/dt = K. (P_m – P), where P is the concentration of protein released and P_m is the maximum protein concentration achievable. K is the first order rate constant and is 0.5 hr^–1 for the system studied. The time required for the release of 90% of the intracellular proteins will be

Measurement of K1a in a bioreactor can be carried out by sodium sulfite oxidation method, that is based on the oxidation of sodium sulfite of sodium sulfate in the presence of a catalyst (Cu⁺⁺ or Co⁺⁺). In a typical experiment, a laboratory fermenter was filled with 5 liter of 0.5 M sodium sulfite solution containing 0.003 MCu⁺⁺ ions and the air was sparged in. After 10 minutes, the air flow was stopped and a 10 ml sample was taken and titrated. The concentration of sodium sulfite in the sample was found to be 0.20 M. The oxygen uptake rate for this aerated system will work out to be

The separation principle of dialysis used in the recovery of fermentation products is

1. Penicillin fermentation is an aerobic process
1. Penicillin biosynthesis affected by phosphate concentration
1. Lysine stimulates penicillin synthesis
1. Penicillin production shows a distinct catabolite repression by glucose

A batch culture fermentation was being conducted with streptomyces rimosus. Analysis of samples collected indicated doubling of cell number per unit time. Youor inference would be that the culture is in the

	dP	= K(P_m - P)
	dt

K =	2.303	log₁₀		100 P
	t			10 P

K =	2.303
	t

t =	2.303	⇒ 4.606 hour
	0.5