Biotechnology Principles and Processes


  1. In genetic engineering, the antibiotics are used









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    Antibiotics are powerful medicines that fight bacterial infections. They either kill bacteria or keep them from reproducing. In genetic engineering, the antibiotics are used as selectable markers.

    Correct Option: A

    Antibiotics are powerful medicines that fight bacterial infections. They either kill bacteria or keep them from reproducing. In genetic engineering, the antibiotics are used as selectable markers.


  1. Biolistics (gene-gun) is suitable for









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    Biolistic it is direct gene transferred method for constructing recombinant DNA. The gene gun was invented by John C. Sanford with Edward Wolf. A gene gun can be used to genetically infect cells or whole organisms with foreign DNA by aiming the barrel of the gun and firing. The microshot projectiles in the biolistic gene gun are made of microscopic (or nano) sized gold or platinum powders. These expensive powders are soaked in DNA or RNA (in raw or plasmid form) that are engineered for insertion into the genome of the cells or organisms under the gun.

    Correct Option: D

    Biolistic it is direct gene transferred method for constructing recombinant DNA. The gene gun was invented by John C. Sanford with Edward Wolf. A gene gun can be used to genetically infect cells or whole organisms with foreign DNA by aiming the barrel of the gun and firing. The microshot projectiles in the biolistic gene gun are made of microscopic (or nano) sized gold or platinum powders. These expensive powders are soaked in DNA or RNA (in raw or plasmid form) that are engineered for insertion into the genome of the cells or organisms under the gun.



  1. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:









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    For gene transfer into the host cell without using vector microparticles made of Tungsten and Gold coated with foreign DNA are bombarded into target cells at a very high velocity.

    Correct Option: D

    For gene transfer into the host cell without using vector microparticles made of Tungsten and Gold coated with foreign DNA are bombarded into target cells at a very high velocity.


  1. Which one is a true statement regarding DNA polymerase used in PCR









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    The name of this DNA polymerase is Taq polymerase extracted from a thermophilic bacteria. It is a relatively thaermostable enzyme which is used in PCR during denaturation, which requires high temperature.

    Correct Option: D

    The name of this DNA polymerase is Taq polymerase extracted from a thermophilic bacteria. It is a relatively thaermostable enzyme which is used in PCR during denaturation, which requires high temperature.



  1. PCR and Restriction Fragment Length Polymorphism are the methods for:









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    NA

    Correct Option: D

    NA