Biotechnology Principles and Processes


  1. The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component (s)?









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    In pBR 322
    ori-represents site of origin or replication rop-represents those proteins that take part in replication of plasmid.
    Hind III, EcoRI- Recoginition sites of Restriction endonucleases ampR and tetR - They are antibiotic resistant gene part

    Correct Option: D

    In pBR 322
    ori-represents site of origin or replication rop-represents those proteins that take part in replication of plasmid.
    Hind III, EcoRI- Recoginition sites of Restriction endonucleases ampR and tetR - They are antibiotic resistant gene part


  1. Genes of interest can be selected from a genomic library by using









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    A hybridization probe is a fragment of DNA of variable length which is used in DNA samples to detect the presence of nucleotide sequence (the DNA target) that are complementary to the sequence in the probe. The probe hybridize to single–stranded DNA whose base sequence allow probe target base-pairing due to complementary between the probe and target.

    Correct Option: C

    A hybridization probe is a fragment of DNA of variable length which is used in DNA samples to detect the presence of nucleotide sequence (the DNA target) that are complementary to the sequence in the probe. The probe hybridize to single–stranded DNA whose base sequence allow probe target base-pairing due to complementary between the probe and target.



  1. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?









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    DNA fragments during gel electrophoresis, separate (resolve) according to their size due to sieving effect provided by agarose gel.

    Correct Option: A

    DNA fragments during gel electrophoresis, separate (resolve) according to their size due to sieving effect provided by agarose gel.


  1. The taq polymerase enzyme is obtained from









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    The Taq polymerase enzyme is obtained from Thermus aquaticus which lives in hot springs.

    Correct Option: A

    The Taq polymerase enzyme is obtained from Thermus aquaticus which lives in hot springs.



  1. Which of the following is not a feature of the plasmids?









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    Plasmid has an extra chromosomal, double stranded circular DNA.

    Correct Option: D

    Plasmid has an extra chromosomal, double stranded circular DNA.