Biotechnology Principles and Processes


  1. Which one of the following is used as vector for cloning genes into higher organisms?









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    Retrovirus as has the ability to transform normal cells into cancerous cells. Hence, it can used as a vector for cloning desirable genes into animal cells.

    Correct Option: D

    Retrovirus as has the ability to transform normal cells into cancerous cells. Hence, it can used as a vector for cloning desirable genes into animal cells.


  1. Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?









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    Palindromic sequences in DNA molecule are group of bases that forms the same sequence when read in both forward and backward direction. In the given question, only option (c) represent a palindromic sequence.

    Correct Option: C

    Palindromic sequences in DNA molecule are group of bases that forms the same sequence when read in both forward and backward direction. In the given question, only option (c) represent a palindromic sequence.



  1. DNA or RNA segment tagged with a radioactive molecule is called









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    DNA or RNA segment tagged with a radioactive molecule is called Probe. They are used to detect the presence of complementary sequences in nucleic acid samples. Probes are used for identification and isolation of DNA and RNA.

    Correct Option: B

    DNA or RNA segment tagged with a radioactive molecule is called Probe. They are used to detect the presence of complementary sequences in nucleic acid samples. Probes are used for identification and isolation of DNA and RNA.


  1. Restriction endonucleases are enzymes which









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    Restriction endonucleases are enzymes that makes cuts at specific positions within the DNA molecule. They acts as molecular scissors. They recognise specific base sequence at palindrome sites in DNA duplex and cut its strands.

    Correct Option: A

    Restriction endonucleases are enzymes that makes cuts at specific positions within the DNA molecule. They acts as molecular scissors. They recognise specific base sequence at palindrome sites in DNA duplex and cut its strands.



  1. Stirred tank bioreactors have been designed for









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    A stirred-tank bioreactor is a cylindrical or curved base to facilitate the mixing of the reaction content. It has been designed for availability of oxygen throughout the process.

    Correct Option: D

    A stirred-tank bioreactor is a cylindrical or curved base to facilitate the mixing of the reaction content. It has been designed for availability of oxygen throughout the process.