Gel electrophoresis is a technique to separation of DNA fragments according to their size. DNA is negatively charged so in gel tank when electricity is passed, DNA move towards positive electrode.

Correct Option: B

Gel electrophoresis is a technique to separation of DNA fragments according to their size. DNA is negatively charged so in gel tank when electricity is passed, DNA move towards positive electrode.

The linking of antibiotic resistance gene with the plasmid vector became possible with DNA ligase. DNA ligase is an enzyme that is able to join together two portions of DNA and therefore plays an important role in DNA repair. DNA ligase is also used in recombinant DNA technology as it ensures that the foreign DNA is bound to the plasmid into which it is incorporated.

Correct Option: A

The linking of antibiotic resistance gene with the plasmid vector became possible with DNA ligase. DNA ligase is an enzyme that is able to join together two portions of DNA and therefore plays an important role in DNA repair. DNA ligase is also used in recombinant DNA technology as it ensures that the foreign DNA is bound to the plasmid into which it is incorporated.

Agrobacterium tumefaciens is the causal agent of crown gall disease (the formation of tumours) in over 140 species of dicot. This disease caused by a DNA plasmid (Tiplasmid) carried by bacterium and transferred to the plant cells. Ti plasmid has widely used in plant engineering as a vector in order to inject gene in host plant to form transgenic plant.

Correct Option: B

Agrobacterium tumefaciens is the causal agent of crown gall disease (the formation of tumours) in over 140 species of dicot. This disease caused by a DNA plasmid (Tiplasmid) carried by bacterium and transferred to the plant cells. Ti plasmid has widely used in plant engineering as a vector in order to inject gene in host plant to form transgenic plant.