Recombinant DNA technology is the process joining together two DNA molecules from two different species that are inserted into a host organism to produce new genetic combination.

Correct Option: A

Recombinant DNA technology is the process joining together two DNA molecules from two different species that are inserted into a host organism to produce new genetic combination.

DNA or RNA segment tagged with a radioactive molecule is called Probe. They are used to detect the presence of complementary sequences in nucleic acid samples. Probes are used for identification and isolation of DNA and RNA.

Correct Option: B

DNA or RNA segment tagged with a radioactive molecule is called Probe. They are used to detect the presence of complementary sequences in nucleic acid samples. Probes are used for identification and isolation of DNA and RNA.

A stirred-tank bioreactor is a cylindrical or curved base to facilitate the mixing of the reaction content. It has been designed for availability of oxygen throughout the process.

Correct Option: D

A stirred-tank bioreactor is a cylindrical or curved base to facilitate the mixing of the reaction content. It has been designed for availability of oxygen throughout the process.

Restriction endonucleases are enzymes that makes cuts at specific positions within the DNA molecule. They acts as molecular scissors. They recognise specific base sequence at palindrome sites in DNA duplex and cut its strands.

Correct Option: A

Restriction endonucleases are enzymes that makes cuts at specific positions within the DNA molecule. They acts as molecular scissors. They recognise specific base sequence at palindrome sites in DNA duplex and cut its strands.

Alternative selectable markers have been developed which differentiate recombinant from non-recombinants on the basis of their ability to produce colour in the presence of chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme β-galactosidase. This results into inactivation of the enzyme, which is referred to as insertional inactivation. The presence of chromogenic substrate give blue coloured colonies of the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the galactosidase and the colonies do not produce any colour, these are identified as recombinant colonies.

Correct Option: D

Alternative selectable markers have been developed which differentiate recombinant from non-recombinants on the basis of their ability to produce colour in the presence of chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme β-galactosidase. This results into inactivation of the enzyme, which is referred to as insertional inactivation. The presence of chromogenic substrate give blue coloured colonies of the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the galactosidase and the colonies do not produce any colour, these are identified as recombinant colonies.