Biotechnology Principles and Processes
- Which of the following is not a feature of the plasmids?
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Plasmid has an extra chromosomal, double stranded circular DNA.
Correct Option: D
Plasmid has an extra chromosomal, double stranded circular DNA.
- What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
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DNA fragments during gel electrophoresis, separate (resolve) according to their size due to sieving effect provided by agarose gel.
Correct Option: A
DNA fragments during gel electrophoresis, separate (resolve) according to their size due to sieving effect provided by agarose gel.
- A gene whose expression helps to identify transformed cell is known as:
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Select table markers in recombinant DNA technology, helps in identification and elimination of non-transform ants and selectively permits the growth of the transform ants.
Correct Option: D
Select table markers in recombinant DNA technology, helps in identification and elimination of non-transform ants and selectively permits the growth of the transform ants.
- The DNA fragments separated on an agarose gel can be visualised after staining with :
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Ethidium bromide (Et Br) is used to stain the DNA fragments and will appear as orange coloured bands when kept under UV light.
Correct Option: C
Ethidium bromide (Et Br) is used to stain the DNA fragments and will appear as orange coloured bands when kept under UV light.
- Commonly used vectors for human genome sequencing are:
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Human genome sequencing is a process that determines the complete DNA sequence of an organism’s genome at a single time. This requires sequencing all of an organism’s chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast. Commonly used vectors for human genome sequencing are BAC (Bacterial artificial chromosomes) and YAC (Yeast artificial chromosomes).
Correct Option: B
Human genome sequencing is a process that determines the complete DNA sequence of an organism’s genome at a single time. This requires sequencing all of an organism’s chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast. Commonly used vectors for human genome sequencing are BAC (Bacterial artificial chromosomes) and YAC (Yeast artificial chromosomes).