Biotechnology Principles and Processes
- A single strand of nucleic acid tagged with a radioactive molecule is called:
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A single strand DNA or RNA tagged with radioactive molecule that is used in hybridization of DNA or RNA is called probe.
Correct Option: D
A single strand DNA or RNA tagged with radioactive molecule that is used in hybridization of DNA or RNA is called probe.
- Which one of the following techniques made it possible to genetically engineer living organism?
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Recombinant DNA technology is the process joining together two DNA molecules from two different species that are inserted into a host organism to produce new genetic combination.
Correct Option: A
Recombinant DNA technology is the process joining together two DNA molecules from two different species that are inserted into a host organism to produce new genetic combination.
- Which one of the following represents a palindromic sequence in DNA?
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A palindromic sequence is a nucleic acid sequence (DNA or RNA) that is the same whether read 5' (five-prime) to 3' (three prime) on one strand or 5' to 3' on the complementary strand with which it forms a double helix.
5' - GAATTC - 3'
3' - CTTAAG - 5'
It is a palindromic sequence of DNA cut by restriction enzyme Eco RI.Correct Option: A
A palindromic sequence is a nucleic acid sequence (DNA or RNA) that is the same whether read 5' (five-prime) to 3' (three prime) on one strand or 5' to 3' on the complementary strand with which it forms a double helix.
5' - GAATTC - 3'
3' - CTTAAG - 5'
It is a palindromic sequence of DNA cut by restriction enzyme Eco RI.
- There is a restriction endonuclease called EcoRI. What does “co” part in it stand for?
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EcoRI is an endonuclease enzyme isolated from strains of E.coli and a part of restriction modified system. So co part stands for coli.
Correct Option: D
EcoRI is an endonuclease enzyme isolated from strains of E.coli and a part of restriction modified system. So co part stands for coli.
- The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component (s)?
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In pBR 322
ori-represents site of origin or replication rop-represents those proteins that take part in replication of plasmid.
Hind III, EcoRI- Recoginition sites of Restriction endonucleases ampR and tetR - They are antibiotic resistant gene partCorrect Option: D
In pBR 322
ori-represents site of origin or replication rop-represents those proteins that take part in replication of plasmid.
Hind III, EcoRI- Recoginition sites of Restriction endonucleases ampR and tetR - They are antibiotic resistant gene part