Biotechnology Principles and Processes


  1. A single strand of nucleic acid tagged with a radioactive molecule is called:









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    A single strand DNA or RNA tagged with radioactive molecule that is used in hybridization of DNA or RNA is called probe.

    Correct Option: D

    A single strand DNA or RNA tagged with radioactive molecule that is used in hybridization of DNA or RNA is called probe.


  1. Which one of the following techniques made it possible to genetically engineer living organism?









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    Recombinant DNA technology is the process joining together two DNA molecules from two different species that are inserted into a host organism to produce new genetic combination.

    Correct Option: A

    Recombinant DNA technology is the process joining together two DNA molecules from two different species that are inserted into a host organism to produce new genetic combination.



  1. Which one of the following represents a palindromic sequence in DNA?









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    A palindromic sequence is a nucleic acid sequence (DNA or RNA) that is the same whether read 5' (five-prime) to 3' (three prime) on one strand or 5' to 3' on the complementary strand with which it forms a double helix.
    5' - GAATTC - 3'
    3' - CTTAAG - 5'
    It is a palindromic sequence of DNA cut by restriction enzyme Eco RI.

    Correct Option: A

    A palindromic sequence is a nucleic acid sequence (DNA or RNA) that is the same whether read 5' (five-prime) to 3' (three prime) on one strand or 5' to 3' on the complementary strand with which it forms a double helix.
    5' - GAATTC - 3'
    3' - CTTAAG - 5'
    It is a palindromic sequence of DNA cut by restriction enzyme Eco RI.


  1. There is a restriction endonuclease called EcoRI. What does “co” part in it stand for?









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    EcoRI is an endonuclease enzyme isolated from strains of E.coli and a part of restriction modified system. So co part stands for coli.

    Correct Option: D

    EcoRI is an endonuclease enzyme isolated from strains of E.coli and a part of restriction modified system. So co part stands for coli.



  1. The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component (s)?









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    In pBR 322
    ori-represents site of origin or replication rop-represents those proteins that take part in replication of plasmid.
    Hind III, EcoRI- Recoginition sites of Restriction endonucleases ampR and tetR - They are antibiotic resistant gene part

    Correct Option: D

    In pBR 322
    ori-represents site of origin or replication rop-represents those proteins that take part in replication of plasmid.
    Hind III, EcoRI- Recoginition sites of Restriction endonucleases ampR and tetR - They are antibiotic resistant gene part