Genetics-ⅱ Miscellaneous
- Si RNA(s) interfere at
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RNA interference (RNAi) also called post transcriptional gene silencing (PTGS), is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. Two types of small ribonucleic acid (RNA) molecules: microRNA (miRNA) and small interfering RNA (siRNA) - are central to RNA interference.
Correct Option: B
RNA interference (RNAi) also called post transcriptional gene silencing (PTGS), is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. Two types of small ribonucleic acid (RNA) molecules: microRNA (miRNA) and small interfering RNA (siRNA) - are central to RNA interference.
- Zinc fingers are characteristics of
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Zinc fingers function as interaction modules that bind DNA, RNA, proteins, or other small, useful molecules.
Correct Option: C
Zinc fingers function as interaction modules that bind DNA, RNA, proteins, or other small, useful molecules.
- RNA is very much susceptible to hydrolysis in alkali because
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While DNA contains deoxyribose, RNA contains ribose (in deoxyribose there is no hydroxyl group attached to the pentose ring in the 2′ position). These hydroxyl groups make RNA less stable than DNA because it is more prone to hydrolysis.
Correct Option: B
While DNA contains deoxyribose, RNA contains ribose (in deoxyribose there is no hydroxyl group attached to the pentose ring in the 2′ position). These hydroxyl groups make RNA less stable than DNA because it is more prone to hydrolysis.
- Which of the following are commonly used as reporter genes?
P. NPTgene
Q. Luciferase gene
R. CFTR gene
S. GFPgene
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Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. Examples include the gene that encodes jellyfish green fluorescent protein (GFP),
which causes cells that express it to glow green under blue light, the enzyme luciferase, which catalyzes a reaction with luciferin to produce light, and the red fluorescent protein from the gene dsRed.Correct Option: A
Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. Examples include the gene that encodes jellyfish green fluorescent protein (GFP),
which causes cells that express it to glow green under blue light, the enzyme luciferase, which catalyzes a reaction with luciferin to produce light, and the red fluorescent protein from the gene dsRed.
- Match the following antibiotics in Group I with their mode of action in Group II
Group I Group II P. Chloramphenicol 1. 0Binds to DNA gyrase 1 Q. Norfloxacin 2. Binds to RNA Polymerase R. Puromycin 3. Inhibits peptidyl transferase S. Rifampicin 4. Mimics aminoacyl tRNA
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Chloramphenicol binds to the peptidyl transferase enzyme to inhibit transfer of the growing polypeptide to the next amino acid occupying the “Acceptor” site. Norfloxacin is a nalidixic acid analogue and one of the most potent DNA gyrase inhibitors. Puromycin is a secondary metabolite of Streptomyces alboniger that blocks protein biosynthesis. Puromycin is a structural analogue of the 32 end of aminoacyl-transfer RNA. Rifampicin inhibits bacterial RNA polymerase by binding to the beta subunit of RNA polymerase.
Correct Option: C
Chloramphenicol binds to the peptidyl transferase enzyme to inhibit transfer of the growing polypeptide to the next amino acid occupying the “Acceptor” site. Norfloxacin is a nalidixic acid analogue and one of the most potent DNA gyrase inhibitors. Puromycin is a secondary metabolite of Streptomyces alboniger that blocks protein biosynthesis. Puromycin is a structural analogue of the 32 end of aminoacyl-transfer RNA. Rifampicin inhibits bacterial RNA polymerase by binding to the beta subunit of RNA polymerase.