Genetics-ⅱ Miscellaneous


  1. Determine the correctness or otherwise of the following Assertion (a) and  Reason (r):
    Assertion (a): IPTG (Isopropylthiogalactoside) is a gratuitous inducer of lac operon.
    Reason (r) : IPTG is an efficient inducer, but not a substrate of lac operon









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    IPTG (I-8000, I-8050) is an artificial inducer of the lac operon. IPTG binds to the lac repressor and inactivates it, preventing its binding to the lac operator and thus inducing the expression of beta-galactosidase. IPTG cannot be hydrolyzed and broken down by the cell and is therefore called a “gratuitous” inducer.

    Correct Option: B

    IPTG (I-8000, I-8050) is an artificial inducer of the lac operon. IPTG binds to the lac repressor and inactivates it, preventing its binding to the lac operator and thus inducing the expression of beta-galactosidase. IPTG cannot be hydrolyzed and broken down by the cell and is therefore called a “gratuitous” inducer.


  1. The full-length coding sequence of an eukaryotic gene was expressed in bacteria and the protein was purified. However, in the functionalassay, no activity was detected for the purified protein. The reason could be









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    Prokaryotes do not carry out the same kinds of post- translational modifications such as glycosylation, phosphorylation as eukaryotes do. This affects a protein’s activity or stability, or at least its response to antibodies.

    Correct Option: A

    Prokaryotes do not carry out the same kinds of post- translational modifications such as glycosylation, phosphorylation as eukaryotes do. This affects a protein’s activity or stability, or at least its response to antibodies.



  1. Which of the following processes require energy?









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    ligation is the ATP dependent procedure. The mechanism of DNA ligase is to form two covalent phosphodiester bonds between 3′ hydroxyl ends of one nucleotide, (“acceptor”) with the 5′ phosphate end of another (“donor”). ATP is required for the ligase reaction.

    Correct Option: A

    ligation is the ATP dependent procedure. The mechanism of DNA ligase is to form two covalent phosphodiester bonds between 3′ hydroxyl ends of one nucleotide, (“acceptor”) with the 5′ phosphate end of another (“donor”). ATP is required for the ligase reaction.


  1. Expression of antisense RNA of ACC synthase in transgenic tomato plants inhibited the synthesis of ethylene resulting in









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    ACC synthase catalyzes the synthesis of ACC, a precursor for ethylene. Ethylene serves as a hormone in plants for stimulating or regulating the ripening of fruit, the opening of flowers, and the abscission (or shedding) of leaves and hence none of the given options is suitable.

    Correct Option: D

    ACC synthase catalyzes the synthesis of ACC, a precursor for ethylene. Ethylene serves as a hormone in plants for stimulating or regulating the ripening of fruit, the opening of flowers, and the abscission (or shedding) of leaves and hence none of the given options is suitable.



  1. What would happen to the mutant cells by ionizing radiation?









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    Ionizing radiation is generally harmful and potentially lethal to living things but can have health benefits in radiation therapy for the treatment
    treatment of cancer and thyrotoxicosis. Its most common impact is the induction of cancer with a latent period of years or decades after exposure. High doses can cause visually dramatic radiation burns, and/or rapid fatality through acute radiation syndrome. Controlled doses are used for medical imaging and radiotherapy. Some scientists suspect that low doses may have a mild hormetic effect that can improve health.

    Correct Option: C

    Ionizing radiation is generally harmful and potentially lethal to living things but can have health benefits in radiation therapy for the treatment
    treatment of cancer and thyrotoxicosis. Its most common impact is the induction of cancer with a latent period of years or decades after exposure. High doses can cause visually dramatic radiation burns, and/or rapid fatality through acute radiation syndrome. Controlled doses are used for medical imaging and radiotherapy. Some scientists suspect that low doses may have a mild hormetic effect that can improve health.