Genetics-ⅱ Miscellaneous


Direction: Normal primary hepatocytes can be artificially immortalized. Certain spontaneous mutants of immortalized hepatocytes are sensitive to ionizing radiation.

  1. Which of the following genes are involved in Immortalization of primary hepatocytes?









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    Telomerase contains a catalytic unit with reverse transcriptase activity and an RNA part that provides template for telomere extension. Telomerase is normally expressed only in stem cells such as those found in hematopoietic tissues and germline cells but is nearly absent in most somatic cells. Induction of telomerase synthesis bypasses normal cellular senesce in cancer cells and endows them with unlimited replicative potential.

    Correct Option: D

    Telomerase contains a catalytic unit with reverse transcriptase activity and an RNA part that provides template for telomere extension. Telomerase is normally expressed only in stem cells such as those found in hematopoietic tissues and germline cells but is nearly absent in most somatic cells. Induction of telomerase synthesis bypasses normal cellular senesce in cancer cells and endows them with unlimited replicative potential.


  1. Expression of which of the following reporter genes does not require addition of specific substrate for detection ?









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    Luciferase bioluminescence color can vary between yellow-green (λmax = 550 nm) to red (λmax = 620) There are currently several different mechanisms describing how the structure of luciferase affects the emission spectrum of the photon and effectively the color of light emitted. One mechanism proposes that the color of the emitted light depends on whether the product is in the keto or enol form. The mechanism suggests that red light is emitted from the keto form of oxyluciferin, while green light is emitted from the enol form of oxyluciferin.

    Correct Option: A

    Luciferase bioluminescence color can vary between yellow-green (λmax = 550 nm) to red (λmax = 620) There are currently several different mechanisms describing how the structure of luciferase affects the emission spectrum of the photon and effectively the color of light emitted. One mechanism proposes that the color of the emitted light depends on whether the product is in the keto or enol form. The mechanism suggests that red light is emitted from the keto form of oxyluciferin, while green light is emitted from the enol form of oxyluciferin.



  1. The enzymes that can be used in 5′ end labeling of DNA are
    (P) alkaline phosphatase
    (Q) DNA ligase
    (R) terminal transferase
    (S) polynucleotide kinase









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    Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. Polynucleotide kinase (or PNK) is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ATP to the free hydroxyl end of the 5’ DNA or RNA.The resulting product could be used to end-label DNA or RNA, or in a ligation reaction.

    Correct Option: A

    Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. Polynucleotide kinase (or PNK) is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ATP to the free hydroxyl end of the 5’ DNA or RNA.The resulting product could be used to end-label DNA or RNA, or in a ligation reaction.


  1. Mobile genetic elements present in human genome are
    P. long interspersed elements (LINEs)
    Q. short interspersed elements (SINEs)
    R. P elements
    S. IS elements









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    Mobile genetic elements are the nucleotide sequences that can change their position in the genome. There are two categories of mobile genetic elements: DNA mobile genetic elements or DNA transposons and retroelements.
    There are 3 kinds of retroelements:
    • LINE( Long Interspersed Nuclear Elements)
    • SINE( Short Interspersed Nuclear Elements)
    • LTR(retrovirus like elements with Long Terminal Repeats)

    Correct Option: B

    Mobile genetic elements are the nucleotide sequences that can change their position in the genome. There are two categories of mobile genetic elements: DNA mobile genetic elements or DNA transposons and retroelements.
    There are 3 kinds of retroelements:
    • LINE( Long Interspersed Nuclear Elements)
    • SINE( Short Interspersed Nuclear Elements)
    • LTR(retrovirus like elements with Long Terminal Repeats)



  1. Presence of CX2–4 CXφX8HX3H sequence in a protein suggest that it is









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    A zinc finger protein is a DNA-binding protein domain consisting of zinc fingers ranging from two in the Drosophila regulator ADR1, the more
    common three in mammalian Sp1 up to nine in TF111A. They occur in nature as the part of transcription factors conferring DNA sequence specificity as the DNA-binding domain.

    Correct Option: C

    A zinc finger protein is a DNA-binding protein domain consisting of zinc fingers ranging from two in the Drosophila regulator ADR1, the more
    common three in mammalian Sp1 up to nine in TF111A. They occur in nature as the part of transcription factors conferring DNA sequence specificity as the DNA-binding domain.