Direction: Normal primary hepatocytes can be artificially immortalized. Certain spontaneous mutants of immortalized hepatocytes are sensitive to ionizing radiation.
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Expression of which of the following reporter genes does not require addition of specific substrate for detection ?
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- Luciferase
- β-Glucuronidase
- β-Glucosidase
- Green fluorescent protein
Correct Option: A
Luciferase bioluminescence color can vary between yellow-green (λmax = 550 nm) to red (λmax = 620) There are currently several different mechanisms describing how the structure of luciferase affects the emission spectrum of the photon and effectively the color of light emitted. One mechanism proposes that the color of the emitted light depends on whether the product is in the keto or enol form. The mechanism suggests that red light is emitted from the keto form of oxyluciferin, while green light is emitted from the enol form of oxyluciferin.
