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The enzymes that can be used in 5′ end labeling of DNA are
(P) alkaline phosphatase
(Q) DNA ligase
(R) terminal transferase
(S) polynucleotide kinase
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- P, S
- R, Q
- P, R
- R, S
Correct Option: A
Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. Polynucleotide kinase (or PNK) is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ATP to the free hydroxyl end of the 5’ DNA or RNA.The resulting product could be used to end-label DNA or RNA, or in a ligation reaction.
