Enzyme Science Miscellaneous

Enzyme Science Miscellaneous

Easy Questions
Moderate Questions
Difficult Questions

Enzyme Science

Enzyme Science Miscellaneous
  1. Using the Hill equation for an enzyme [S]0 = (v0 Km/ Vmax– v0)1/n and the plot of log10(v0/Vmax – v0) vs log 10 [S]0 one can find out
    (P) Vmax form the intercept on the ordinate
    (Q) Km form the intercept on the ordinalte
    (R) ‘n’ from the slope
    (S) Km from the intercept on the abscissa








  1. View Hint View Answer Discuss in Forum

    Vm can be plotted and calculated from the intercept on the ordinate while Km will be given by Hill equation from the intercept of the abscissa.

    Correct Option: D

    Vm can be plotted and calculated from the intercept on the ordinate while Km will be given by Hill equation from the intercept of the abscissa.

  1. Match the following products with their starting substrates
    3. barleyD. lager
    A. Sake 1. apple juice
    B. cider2. grape juice
    C. wine
    4. rice








  1. View Hint View Answer Discuss in Forum

    Sake or saké is an alcoholic beverage of that is made from fermented rice. Cider is a fermented alcoholic beverage made from fruit juice, most commonly and traditionally apple juice. Grape juice is obtained from crushing and blending grapes into a liquid. The juice is fermented and made into wine, brandy or vinegar. Barley is used as a source of fermentable material for beer called lager and certain distilled beverages.

    Correct Option: A

    Sake or saké is an alcoholic beverage of that is made from fermented rice. Cider is a fermented alcoholic beverage made from fruit juice, most commonly and traditionally apple juice. Grape juice is obtained from crushing and blending grapes into a liquid. The juice is fermented and made into wine, brandy or vinegar. Barley is used as a source of fermentable material for beer called lager and certain distilled beverages.

  1. Immobilization of enzymes
    (P) increases the specificity of the enzyme for its reactants
    (Q) facilitates reuse of the enzyme in batch reactions
    (R) makes it unsuitable for its use in a continuous reactor system
    (S) decreases the operational cost of the industrial process








  1. View Hint View Answer Discuss in Forum

    The immobilized enzyme is easily removed from the reaction making it easy to recycle the biocatalyst. Immobilized enzymes typically have greater thermal and operational stability than the soluble form of the enzyme. This reduces the operational cost.

    Correct Option: A

    The immobilized enzyme is easily removed from the reaction making it easy to recycle the biocatalyst. Immobilized enzymes typically have greater thermal and operational stability than the soluble form of the enzyme. This reduces the operational cost.

  1. Immobilization of enzymes using entrapment method requires
    (P) photosensitive polyethylene glycol dimethacrylate
    (Q) CNBr activation of sepharose
    (R) polyfunctional reagent like hexamethylene diisocyanate
    (S) radiation of polyvinyl alcohol








  1. View Hint View Answer Discuss in Forum

    Photosensitive Polyethylene glycol dimethy-lacrylate is used in free radical copolymer crosslinking reactions which cross links enzyme with matrix and hence used in immobilzation of enzymes. Polyvinyl alcohol is used for formation of spherical structured beads in immobilisation process but after the process is over it needs to be degraded and for this purpose gamma radiation induced degradation of polyvinyl alcohol is carried out.

    Correct Option: C

    Photosensitive Polyethylene glycol dimethy-lacrylate is used in free radical copolymer crosslinking reactions which cross links enzyme with matrix and hence used in immobilzation of enzymes. Polyvinyl alcohol is used for formation of spherical structured beads in immobilisation process but after the process is over it needs to be degraded and for this purpose gamma radiation induced degradation of polyvinyl alcohol is carried out.

Direction: Enzyme X converts substrates S1 and S2 (which are similar but not
identical) to products P1 and P2 respectively

  1. What would happen if enzyme X is incubated with a mixture of 0.1 mM of S1 and S2?








  1. View Hint View Answer Discuss in Forum

    Because this enzyme has a high KM, less substrate is converted into product.

    Correct Option: C

    Because this enzyme has a high KM, less substrate is converted into product.