6. The fold purification for each step is

1. 1. P – 0.1, Q – 0.66, R – 0.84, S – 1.26

   
   
   

   2. P – 1.0, Q – 0.52, R – 0.67, S – 0.8

   
   
   

   3. P – 1, Q – 6.6, R – 8.4, S – 12.6

   
   
   

   4. P – 100, Q – 66, R – 84, S – 12

   
   
   

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   NA

   Correct Option: C

   NA

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7. The yield (%) for each step is
   

1. 1. P – 10, Q – 7.2, R – 4.5, S – 2.0

   
   
   

   2. P – 34, Q – 24, R – 15, S – 1

   
   
   

   3. P – 3.4, Q – 2.4, R – 1.5, S – 0.1

   
   
   

   4. P – 100, Q – 71, R – 44, S – 29

   
   
   

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   NA

   Correct Option: D

   NA

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8. The molar concentration of the repressor in the cell is
   

1. 1. 1.1 nM

   
   
   

   2. 1 nM

   
   
   

   3. 10 nM

   
   
   

   4. 100 nM

   
   
   

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   NA

   Correct Option: D

   NA

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9. Therefore the lac-operon is
   

1. 1. repressed and can only be induced with lactose.

   
   
   

   2. repressed and cannot be induced with lactose.

   
   
   

   3. not repressed.

   
   
   

   4. expressed only when glucose and lactose are present.

   
   
   

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   NA

   Correct Option: B

   NA

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10. One unit of glucoamylase enzyme activity is defined as the amount of enzyme required to produce (imol of glucose per min in a 4% solution of Lintner starch at pH 4.5 and 60°C. If in a reaction mixture with 1ml of the crude enzyme preparation containing 8 mg protein and 9 ml of 4.44% starch, 0.6 µmc of glucose/ml-min is produced, what will be the specific activity of the crude enzyme preparation?
    

1. 1. 1 unit/mg protein

   
   
   

   2. 1.5 units/mg protein

   
   
   

   3. 0.25 units/mg protein

   
   
   

   4. 0.75 units/mg protein

   
   
   

   5. None of these

   
   
   

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   NA

   Correct Option: E

   NA

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