1. The molar concentration of the repressor in the cell is
   

1. 1. 1.1 nM

   
   
   

   2. 1 nM

   
   
   

   3. 10 nM

   
   
   

   4. 100 nM

   
   
   

---

1. View Hint View Answer Discuss in Forum

   NA

   Correct Option: D

   NA

---

2. The fold purification for each step is

1. 1. P – 0.1, Q – 0.66, R – 0.84, S – 1.26

   
   
   

   2. P – 1.0, Q – 0.52, R – 0.67, S – 0.8

   
   
   

   3. P – 1, Q – 6.6, R – 8.4, S – 12.6

   
   
   

   4. P – 100, Q – 66, R – 84, S – 12

   
   
   

---

1. View Hint View Answer Discuss in Forum

   NA

   Correct Option: C

   NA

---

---

3. The yield (%) for each step is
   

1. 1. P – 10, Q – 7.2, R – 4.5, S – 2.0

   
   
   

   2. P – 34, Q – 24, R – 15, S – 1

   
   
   

   3. P – 3.4, Q – 2.4, R – 1.5, S – 0.1

   
   
   

   4. P – 100, Q – 71, R – 44, S – 29

   
   
   

---

1. View Hint View Answer Discuss in Forum

   NA

   Correct Option: D

   NA

---

4. An enzymatic reaction is described by the following rate expression.
   

   V =	VμS
   	Kas + S + S²ks

   
   Which one of the following curves represents this expression?

1. 1. 

   
   
   

   2. 

   
   
   

   3. 

   
   
   

   4. 

   
   
   

---

1. View Hint View Answer Discuss in Forum

   NA

   Correct Option: A

   NA

---

---

5. Therefore the lac-operon is
   

1. 1. repressed and can only be induced with lactose.

   
   
   

   2. repressed and cannot be induced with lactose.

   
   
   

   3. not repressed.

   
   
   

   4. expressed only when glucose and lactose are present.

   
   
   

---

1. View Hint View Answer Discuss in Forum

   NA

   Correct Option: B

   NA

---