Bioenergetics and Metabolism Miscellaneous


Bioenergetics and Metabolism Miscellaneous

Bioenergetics and Metabolism

  1. An alternative to glycolysis pathway is









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    The pentose phosphate pathway (PPP) is a process of glucose turnover that produce NaDPH as reducing equivalents and pentose as essential part of nucleotides. There are two different phases in a pathway. One is irreversible oxidase phase in which glucose-6-phosphate is converted to ribulose-6-phosphate by oxidative decarboxylation and NaDPH is generated. The other is reversible non- oxidative phase in which phosphorylated sugars are interconverted to generate xylulose -5p, ribulose-5p and ribose-5p. Phosphoribosyl pyrophosphate formed from ribose-5p is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway main purpose is to regenerate
    NaDP+ through an oxidation/reduction reaction. NaDPH is used for reductive reaction in anabolism, especially in fatty acid synthesis.

    Correct Option: B

    The pentose phosphate pathway (PPP) is a process of glucose turnover that produce NaDPH as reducing equivalents and pentose as essential part of nucleotides. There are two different phases in a pathway. One is irreversible oxidase phase in which glucose-6-phosphate is converted to ribulose-6-phosphate by oxidative decarboxylation and NaDPH is generated. The other is reversible non- oxidative phase in which phosphorylated sugars are interconverted to generate xylulose -5p, ribulose-5p and ribose-5p. Phosphoribosyl pyrophosphate formed from ribose-5p is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway main purpose is to regenerate
    NaDP+ through an oxidation/reduction reaction. NaDPH is used for reductive reaction in anabolism, especially in fatty acid synthesis.


  1. In N-linked glycosylation, the oligosaccharide chain is attached to protein by









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    All N-linked glycans are derived from a common 14-sugar oligosaccharide synthesized in the ER, which is attached co-translationally to a protein while this is being translated inside the reticulum. The process of the synthesis of this glycan, known as Synthesis of the N-glycan precursor or LLO, constitutes one of the most conserved pathways in eukaryotes, and has been also observed in some eubacteria. The attachment usually happens on an asparagine residue within the consensus sequence asparagine-X-threonine by an complex called oligosaccharyl transferase(OST). After being attached to an unfolded protein, the glycan is used as a label molecule in the folding process (also known as Calnexin/Calreticulin cycle).

    Correct Option: A

    All N-linked glycans are derived from a common 14-sugar oligosaccharide synthesized in the ER, which is attached co-translationally to a protein while this is being translated inside the reticulum. The process of the synthesis of this glycan, known as Synthesis of the N-glycan precursor or LLO, constitutes one of the most conserved pathways in eukaryotes, and has been also observed in some eubacteria. The attachment usually happens on an asparagine residue within the consensus sequence asparagine-X-threonine by an complex called oligosaccharyl transferase(OST). After being attached to an unfolded protein, the glycan is used as a label molecule in the folding process (also known as Calnexin/Calreticulin cycle).



  1. Match the products in Group I with their respective organisms in Group II.
    Group I
    P. Glycerol
    Q. Glutamic acid
    R. Curdlan
    S. Amphotericin B
    Group II
    1. Corynebacterium glutamicum
    2. Alcaligenes faecalis
    3. Dunaliella salina
    4. Streptomyces nodosus









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    Glycerol is produced by Dunaliella salina. Glutamic acid is produced by corynebacterium glutamicum. Curdlan is synthesised by Alcaligenes faecalis . AmphotericinB is apolyene antifungal drug, often used intravenously for systemic fungal infections. It was originally extracted from Streptomyces nodosus, a filamentous bacterium.

    Correct Option: C

    Glycerol is produced by Dunaliella salina. Glutamic acid is produced by corynebacterium glutamicum. Curdlan is synthesised by Alcaligenes faecalis . AmphotericinB is apolyene antifungal drug, often used intravenously for systemic fungal infections. It was originally extracted from Streptomyces nodosus, a filamentous bacterium.


  1. Match items in Group I with Group II.
    Group I
    P. Glycolytic pathway
    Q. Eukaryotic oxidative metabolism
    R. Glyoxylate cycle
    S. Calvin cycle
    Group II
    1. Chloroplast
    2. Glyoxysomes
    3. Mitochondria
    4. Cytosol









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    In Plants, Cholroplast is the host for Glycolytic pathway. Oxidative oxidation in eukaryotes takes place at the Glyoxysomes. Mitochondria is the house of glyoxalate cycle in eukaryotes while cytosol host the Calvin cycle.

    Correct Option: C

    In Plants, Cholroplast is the host for Glycolytic pathway. Oxidative oxidation in eukaryotes takes place at the Glyoxysomes. Mitochondria is the house of glyoxalate cycle in eukaryotes while cytosol host the Calvin cycle.



  1. During lactic acid fermentation, net yield of ATP and NADH per mole of glucose is









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    Lactic acid fermentation is caused by some fungi and bacteria. The most important lactic acid producing bacteria is lactobacillus. It is used to convert lactose lactic acid in yogurt production. In lactic acid fermentation, NADH is oxidized into 2 molecules of NAD+ with a net gain of 2ATP and 2lactate.
    Pyruvate + 2NADH ⇒ Lactic Acid + 2NAD+ + 2ATP.

    Correct Option: B

    Lactic acid fermentation is caused by some fungi and bacteria. The most important lactic acid producing bacteria is lactobacillus. It is used to convert lactose lactic acid in yogurt production. In lactic acid fermentation, NADH is oxidized into 2 molecules of NAD+ with a net gain of 2ATP and 2lactate.
    Pyruvate + 2NADH ⇒ Lactic Acid + 2NAD+ + 2ATP.