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Bioenergetics and Metabolism Miscellaneous

Bioenergetics and Metabolism

  1. In N-linked glycosylation, the oligosaccharide chain is attached to protein by
    1. asparagine
    2. arginine
    3. serine
    4. threonine
Correct Option: A

All N-linked glycans are derived from a common 14-sugar oligosaccharide synthesized in the ER, which is attached co-translationally to a protein while this is being translated inside the reticulum. The process of the synthesis of this glycan, known as Synthesis of the N-glycan precursor or LLO, constitutes one of the most conserved pathways in eukaryotes, and has been also observed in some eubacteria. The attachment usually happens on an asparagine residue within the consensus sequence asparagine-X-threonine by an complex called oligosaccharyl transferase(OST). After being attached to an unfolded protein, the glycan is used as a label molecule in the folding process (also known as Calnexin/Calreticulin cycle).



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