Bioenergetics and Metabolism Miscellaneous
- The reactions leading to the formation of amino acids from the TCA cycle intermediates are
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Transamination refers to the transfer of an amine group from one molecule to another. This reaction is catalyzed by a family of enzymes called transaminases. Actually, the transamination reaction results in the exchange of an amine group on one acid with a ketone group on another acid. It is analogous to a double replacement reaction. The most usual and major keto acid involved with transamination reactions is alpha-ketoglutaric acid, an intermediate in the citric acid cycle. A specific example is the transamination of alanine to make pyruvic acid and glutamic acid.
Correct Option: C
Transamination refers to the transfer of an amine group from one molecule to another. This reaction is catalyzed by a family of enzymes called transaminases. Actually, the transamination reaction results in the exchange of an amine group on one acid with a ketone group on another acid. It is analogous to a double replacement reaction. The most usual and major keto acid involved with transamination reactions is alpha-ketoglutaric acid, an intermediate in the citric acid cycle. A specific example is the transamination of alanine to make pyruvic acid and glutamic acid.
- The standard free energy change (?G'°) for ATP hydrolysis is –30 kJ.mole–1. The in vivo concentrations of ATP, ADP and Pi in E. coli are 7.90, 1.04 and 7.90 mM, respectively. When E. coli cells are cultured at 37°C, the free energy change (?G) for ATP hydrolysis in vivo is ______ kJ. mole–1.
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ATP + H2O → ADP + Pi
∆ G° = – 30 Kj/mol
Τ = 37°C∆G = ∆G° + RT ln [ADP][P2] [ATP] = –30 + 8.314 × 10–3 × (37 + 273) In (1.04 × 10³ × 7.90 × 10³) (7.90 × 10-3)
= –30 – 17.70 = – 47.70 KJ/molCorrect Option: C
ATP + H2O → ADP + Pi
∆ G° = – 30 Kj/mol
Τ = 37°C∆G = ∆G° + RT ln [ADP][P2] [ATP] = –30 + 8.314 × 10–3 × (37 + 273) In (1.04 × 10³ × 7.90 × 10³) (7.90 × 10-3)
= –30 – 17.70 = – 47.70 KJ/mol
