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  1. Evaluate the Michaelis constant for the following lipase catalyzed trans-esterification reaction for the production of biodiesel

    where, k1 = 3 × 108 M–1 s–1; k = 4 × 10³ s–1 and k2 = 2 × 10³ s–1
    1. 4.2 × 10–3 M
    2. 14.0 × 10–4 M
    3. 6.4 × 10–6 M
    4. 1.4 × 10–4 M
Correct Option: D

Km =
K1 + K2
K1

=
4 × 104S-1 + 2 × 108 S-1
3 × 108M-1S-1

=
4200
3 × 108

= 1.4 × 10-4M



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