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  1. Which of the following techniques is best suited for immobilizing an affinity ligand?
    1. Physical adsorption
    2. Gel entrapment
    3. Cross-linking with a polymer
    4. Covalent linkage to a spacer arm
Correct Option: D

Affinity chromatography utilizes the specific interactions between two molecules for the purification of a target molecule. A ligand having affinity for a target molecule is covalently attached to an insoluble support and functions as bait for capturing the target from complex solutions. The affinity ligand can be any molecule that will bind the target without also binding other molecules in the solution.



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