Direction: An enzyme (24000 Da) undergoes first-order deactivation kinetics while catalyzing a reaction according to Michaelis-Menten kinetics (Km= 10–4 M). The enzyme has a turnover number if 104 molecules substrate/min-(molecule enzyme) and a deactivation constant (kd) of 0.1 min–1 at the reaction conditions. The reaction mixture initially contains 0.6 mg/l of active enzyme and 0.02 M of the substrate.
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The time required to convert 10% of the substrate will be rapproximately
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- 16 min
- 24 min
- 32 min
- 8 min
- NA
Correct Option: E
NA