Direction: A Nick Translation reaction in a final volume of 100µl was carried out by using 25 µCi of labeled [α-32P]–dCTP for labeling a 1.2 Kb λ-Interferon DNA fragment.
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After completion of Nick translation reaction, 10 µl of reaction was spotted on a glass-fiber filter that upon counting resulted into 4.2 × 104 cpm in reaction. Another 10 µl was processed for TCA precipitation to determine redioisotope incorporation. The TCA precipitated sample gave 2.94 × 104 cpm. What is the percent of [α-32P]-dCTP incorporation into the DNA sample?
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- 40%
- 50%
- 60%
- 70%
Correct Option: D
% incorporation is calculated by incorporation into precipitated form. (The polymerized form will precipitate).
| = | ||
| total epm |
| = | × 100 ≅ 70% | |
| 4.2 × 104 emp |
