Direction: A Nick Translation reaction in a final volume of 100µl was carried out by using 25 µCi of labeled [α-32P]–dCTP for labeling a 1.2 Kb λ-Interferon DNA fragment.
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If 2.94 × 104 cpm of TCA precipitable counts of the 10 µl sample were taken from 1/10 dilutioa of the 100µl Nick Translation reaction containing 1 µg of γ-Interferon DNA, what is the specific activity of the labeled product?
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- 1.47 × 106 cpm/µg
- 1.47 × 107 cpm/µg
- 2.94 × 106 cpm/µg
- 2.94 × 107 cpm/µg
Correct Option: D
2.94 × 105 cpm is from 0.1 dilution of 10 µl sample. Thus, 10 µl sample will have 2.94 × 106 cpm but, this 10 µl sample is taken from 100 µl which contain 1 µg of λ-interferon. So, 100 µl of 1 µg interferon sample will have 2.94 × 107 cmp/µg.
