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The interaction between an antigen (Ag) and a single-chain antibody (Ab) was studied using Scatchard analysis. The result is shown below.
The affinity of interaction and the total concentration of antibody, respectively, can be determined from
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- slope and Y-intercept
- Y-intercept and slope
- X-intercept and slope
- slope and X-intercept
Correct Option: D
Scatchard analysis is plot of ratio of concentration of bound ligand to unbound ligand verses the bound ligand concentration. The slope = affinity for ligand binding and Y intercept = total concentration of antibody.
| = - | ka + nka | |||
| [Ag] | [Ag] |
Then compare with equation y = mx + c
