Identify the statement that is NOT applicable to an enzyme

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Identify the statement that is NOT applicable to an enzyme catalyzed reaction

1. Enzyme catalysis involves propinquity effects
2. The binding of substrate to the active site causes a strain in the substrate
3. Enzymes do not accelerate the rate of reverse reaction
4. Enzyme catalysis involves acid-base chemistry

Correct Option: C

Enzymes accelerate the rate of the reverse reaction as well as the forward reaction, it would be helpful to ignore any back reaction by which E1P might form ES. The velocity of this back reaction would be given by v = k_–2[E][P].