Direction: A double reciprocal plot was created from the specific growth rate and limiting-substrate concentration data obtained from a chemostat experiment. A linear regression gave values of 1.25 hr and 100 mg-hr-l–1 for the intercept and slope, respectively.
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The same culture (with the µm and Ks values as computed above) is cultivated in a 10-litre chemostat being operated with a 50 ml/min sterile feed containing 50 g/l of substrate. Assuming an overall yield coefficient of 0.3 g-DCW/g-substrate, the respective values of the outlet biomass and substrate concentration are
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- 15 g/l, 48 mg/l
- 15 g/l, 0.48 g/l
- 48 g/l, 15 g/l
- 4.8 g/l, 4.8 g/l
Correct Option: A
NA
