Direction: An aliquot of competent E. coli cells were used for determination of cell density by plate count method and another aliquot was used for transformation by plasmid DNA
-
Isolated plasmid DNA (5µg) was used for transformation of 100 µl competent E. coli cells to which 900 µl of SOC medium was added. An aliquot of 50ul was plated on a selective plate. After overnight incubation, 300 colonies were observed. Calculate the efficiency of transformation and the percentage of transformed cells per ml of parent culture
-
- 6.0 × 105 colonies per µg of plasmid DNA, 0.01%
- 1.2 × 105 colonies per µg of plasmid DNA, 0.02%
- 1.2 × 106 colonies per µg of plasmid DNA, 0.008%
- 6.0 × 106 colonies per µg of plasmid DNA, 0.01%
Correct Option: B
NA
