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A protein binds to phosphocellulose column at pH 7.0 and elutes at pH 8.0. If the protein has to be further purified on a DEAE Sephacel column, the binding buffer should have a pH of
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- 5
- 6
- 7
- 8
Correct Option: C
If the protein is most stable at pH's above its pI, then an anion-exchange gel matrix should be used. If it is most stable below its pI, then a cation-exchange gel matrix should be used. Here the protein eluted at pH 8 so it would take pH7 to bind in DEAE column.
