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A T-flask is seeded with 105 anchorage-dependent cells. The available area of the T-flask is 25 cm2 and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 µm × 2 µm. If the cells grow to monolayer confluence after 50 h, the growth rate in number of cells/(cm2.h) is ____ × 105 .
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Correct Option: B
Given, Seeding of T-flask with = 105 no. of anchorage dependent cells
Area of flask available = 25 cm2 and volume of flask = 25 mL
Size of cells = 5µm × 2µm
Area of the T-flask covered by a single cell = 10 µm2 = 10 × 10-8 cm2
Now, anchorage dependent cells multiply every hour per unit area
So, the growth rate of the cells = 2 × 105 cells/cm2h
