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Consider a continuous culture provided with a sterile feed containing 10 mM glucose. The steady state cell density and substrate concentration at three different dilution rates are given in the table below.
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- 0.795 to 0.805
- 0.395 to 0.405
- 7.95 to 8.05
- None of the above
Correct Option: A
At dilution rate = 5h-1, cell density = 0. This means cell wash out occurs at this dilution rate
The mass balance on the microorganisms in a CSTR of constant volume is :
[Rate of accumulation of cells, g/s] = [Rate of cells entering, g/s] – [Rate of cells leaving, g/s] + [Net rate of generation of live cells, g/s]
So, maximum specific growth rate µmax = 0.8 h-1
