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Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given N = rotations/time, D = diameter of impeller)
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- π ND
- π N2 D
- π ND2
- none of these
Correct Option: A
Shear speed of an impeller along with the diameter of the impeller blade have profound impact on the shear stress generated in the bioreactor system. Mammalian cells are very shear sensitive. So, for scale up of a small scale bioreactor where mammalian cells are used as feed, the major parameters to be considered would be the shear speed and agitator diameter. This is because, while scaling up, impeller speed has to be considered for controlling the shear speed which is given by : TI = πND
