Direction: Lignocellulosic biomass was subjected to microbial composting. The microbial consortium produced an extracellular enzyme xylanase, which was a glycoprotein having a molecular weight of 68 kDa and a positive charge. An aqueous extract of the enzyme could be easily prepared from the compost.
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If Con A sepharose column was used for the purification of enzyme, then separation would be based on
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- molecular exclusion
- affinity binding
- ion exchange
- hydrophobic interaction
Correct Option: B
Con A has varying affinities for various polypeptides and hence affinity binding will be used.
