Direction: Lignocellulosic biomass was subjected to microbial composting. The microbial consortium produced an extracellular enzyme xylanase, which was a glycoprotein having a molecular weight of 68 kDa and a positive charge. An aqueous extract of the enzyme could be easily prepared from the compost.
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What techniques would you recommend for confirming the molecular weight of the purified enzyme?
(P) Isoelectric focusing
(Q) SDS-PAGE
(R) Native PAGE
(S) Gel filtration
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- P, Q
- Q, S
- R, S
- P, S
Correct Option: B
Gel filtration is a separation based on size and same is the purpose of SDS-PAGE i.e. to separate proteins according to their size, and no other physical feature.
